Substance profile
Subtilisin
Subtilisin is a light-colored, free-flowing powders.
01 · Identification
What Subtilisin is
Subtilisin is a light-colored, free-flowing powders. Its molecular formula is C45H68N10O15 and its molecular weight is 989.1 g/mol.
Under the GHS it carries the signal word Danger.
02 · Identifiers
Subtilisin identifiers and molecular data
Subtilisin CAS number and EC number
Subtilisin molecular formula and molecular weight
03 · GHS classification
Subtilisin hazard classification
Harmonised classification under CLP Annex VI. This is the legally binding classification in the EU; other jurisdictions may differ.
Subtilisin pictograms and signal word
Subtilisin hazard class and category
| Hazard class | Category | From code | Pictogram |
|---|---|---|---|
| Skin corrosion/irritation GHS chapter 3.2 | Category 2 | H315 | GHS07 |
| Serious eye damage/eye irritation GHS chapter 3.3 | Category 1 | H318 | GHS05 |
| Respiratory sensitisation GHS chapter 3.4 | Category 1 or 1A or 1B | H334 | GHS08 |
| Specific target organ toxicity - single exposure GHS chapter 3.8 | Category 3 | H335 | GHS07 |
Where two values are shown with “or”, the hazard statement is shared by more than one category or by more than one class, and the code alone does not say which. H272, for example, belongs to oxidising liquids and to oxidising solids — the physical state decides. The binding answer for a specific entry is in the CLP Annex VI table itself.
Subtilisin hazard statements (H-codes)
| Code | Hazard statement |
|---|---|
| H335 | May cause respiratory irritation. |
| H315 | Causes skin irritation. |
| H318 | Causes serious eye damage. |
| H334 | May cause allergy or asthma symptoms or breathing difficulties if inhaled. |
This page lists only the codes assigned to Subtilisin. The full reference lives on the hazard statements hub, the precautionary statements hub and the GHS pictograms hub.
Safety Data Sheet
Getting an SDS for Subtilisin
A Safety Data Sheet is written for a product, not for a substance: the supplier of the grade you actually buy issues it, so two suppliers of Subtilisin issue two different sheets. That is why there is no single authoritative SDS for a substance to download — what there is, is the job of collecting the sheets for the products you hold and keeping them current as suppliers revise them.
04 · Precautionary statements
Subtilisin precautionary statements
CLP Annex VI assigns 16 precautionary statements to Subtilisin. They are grouped the way a label groups them: what to do before handling, what to do when something has already happened, how to store it and how to dispose of it.
Prevention
What to do before and during handling so the hazard never happens.
Response
What to do once exposure, spillage or fire has already happened.
Reading the notation. … is a blank the supplier fills in — not a truncated sentence.· [square brackets] mark a part printed only when it applies.
05 · Names in the regulation
Subtilisin name in 23 EU languages
CLP Annex VI Part 3 is published in every language edition of the regulation, and the identification column is written out in each of them. Nothing below is translated by us: each line is the name as that edition prints it. Under Art. 18(2) the substance name from Annex VI is a lawful product identifier, so this is the wording to put on a label going to that market — and where the cell lists several designations, any one of them qualifies.
| Language | Name in CLP Annex VI Part 3 |
|---|---|
| Bulgarian български | субтилизин |
| Spanish español | Subtilisina |
| Czech čeština | subtilisin |
| Danish dansk | subtilisin |
| German Deutsch | Subtilisin |
| Estonian eesti | subtilisiin |
| Greek ελληνικά | σουμπτιλισίνη |
| English English | subtilisin |
| French français | subtilysine |
| Croatian hrvatski | subtilizin |
| Italian italiano | subtilisina |
| Latvian latviešu | subtilizīns |
| Lithuanian lietuvių | subtilizinas |
| Hungarian magyar | szubtilizin |
| Maltese Malti | subtiliżina |
| Dutch Nederlands | subtilisine |
| Polish polski | subtylisyna |
| Portuguese português | subtilisina |
| Romanian română | subtilizină |
| Slovak slovenčina | subtilizín |
| Slovenian slovenščina | subtilizin |
| Finnish suomi | subtilisiini |
| Swedish svenska | subtilisin |
All 23 language editions of Annex VI carry this entry. Irish is the twenty-fourth official EU language and has no line here for a different reason: no consolidated CLP text has ever been published in Irish, so there is no Irish edition of Annex VI to quote a name from. Source: consolidated CLP, CELEX 02008R1272, language editions. © European Union, https://eur-lex.europa.eu — only the electronic Official Journal of the European Union is authentic.
06 · Label elements
Subtilisin GHS label elements
Everything CLP Annex VI fixes for Subtilisin, laid out the way it appears on a label. The greyed lines are the parts only the supplier can fill in.
Danger
Subtilisin
- May cause respiratory irritation.
- Causes skin irritation.
- Causes serious eye damage.
- May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- Obtain special instructions before use.
- Avoid breathing dust/fume/gas/mist/vapours/spray.
- Wash … thoroughly after handling.
- Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/…
- [In case of inadequate ventilation] wear respiratory protection.
- IF ON SKIN:
- IF INHALED:
- IF IN EYES:
- Immediately call a POISON CENTER/doctor/…
- Call a POISON CENTER/doctor/…
- Get medical advice/attention.
- If skin irritation occurs:
- Remove contact lenses, if present and easy to do. Continue rinsing.
- Remove person to fresh air and keep comfortable for breathing.
- If experiencing respiratory symptoms:
- Wash with plenty of water/…
Supplier name, address and telephone number· Product identifier·Nominal quantity
Need this as an actual label? Open Subtilisin in the GHS label constructor →
07 · Health hazards
Subtilisin health hazards and exposure limits
Subtilisin hazard summary
Occupational asthma reported in detergent industry; Study of 1642 workers found prevalence of 3.2% (over 7 years); [Malo] Causes washing powder lung, a form of hypersensitivity pneumonitis from exposure to detergents that contain enzymes produced by the nonthermophilic bacteria (Bacillus subtilis & B. cereus); [Rosenstock, p. 346] See ENZYMES.
Source: Haz-Map, Information on Hazardous Chemicals and Occupational Diseases
Subtilisin occupational exposure limits
| Limit | Value | Source |
|---|---|---|
| PEL | none See Appendix G | The National Institute for Occupational Safety and Health (NIOSH) |
| REL | 0.00006 mg/m³ [60 minutes] | Occupational Safety and Health Administration (OSHA) |
| REL | ST 0.00006 mg/m3 [60-minute] | The National Institute for Occupational Safety and Health (NIOSH) |
| IDLH | N.D. | The National Institute for Occupational Safety and Health (NIOSH) |
These are United States values. The European Union sets its own indicative and binding limits, and the figure that applies at a given workplace is the one in the local regulation — not the one shown here.
08 · Physical and chemical properties
Subtilisin properties
Every value is shown with the conditions it was measured under and the source it came from. Where sources disagree, all values are listed rather than one being picked — the difference is usually a different form of the substance, not an error.
Subtilisin solubility
| Property | Value | Conditions | Source |
|---|---|---|---|
| Solubility | Show good solubility but little stability in water | HSDB |
Subtilisin vapor pressure
| Property | Value | Conditions | Source |
|---|---|---|---|
| Vapor pressure | 0 mmHg | HSDB | |
| 0 mmHg | approx | NIOSH |
Values compiled from PubChem Laboratory Chemical Safety Summary. Temperatures converted to Fahrenheit for reference.
09 · Handling and storage
Subtilisin storage and handling
Subtilisin storage conditions
Alcalase 2.4L (Prod Code P4860) is is stable for at least one year when stored at 2-8 °C and 3 months when stored at 25 °C.
Source: Hazardous Substances Data Bank (HSDB)
Subtilisin incompatible materials
None reported
Source: The National Institute for Occupational Safety and Health (NIOSH)
Planning storage for a mix of chemicals? Check Subtilisin in the storage compatibility matrix →
10 · Questions
Subtilisin — frequently asked questions
What is the CAS number of Subtilisin?
The CAS number of Subtilisin is 9014-01-1. Its EC number is 232-752-2.
What is the chemical formula of Subtilisin?
The molecular formula of Subtilisin is C45H68N10O15 and its molecular weight is 989.1 g/mol.
What does a Subtilisin label have to show?
Under CLP Annex VI the label for Subtilisin carries the pictograms Health hazard, Corrosion and Exclamation mark and the signal word is Danger.
Is Subtilisin flammable?
CLP Annex VI assigns Subtilisin no flammability hazard class. That is not a statement that it cannot burn: the harmonised list only records the hazards that were assessed for it.
Is Subtilisin a carcinogen?
CLP Annex VI records no harmonised carcinogenicity classification for Subtilisin. Annex VI is a list of agreed classifications, not a list of substances found safe, so this is an absence of a classification rather than a clean bill of health.
What is the exposure limit for Subtilisin?
The US occupational exposure limit reported for Subtilisin is none See Appendix G (The National Institute for Occupational Safety and Health (NIOSH)). Limits differ by country: the EU sets its own indicative and binding values, and the figure that applies at a given workplace is the one in the local regulation.
How should Subtilisin be stored?
Alcalase 2.4L (Prod Code P4860) is is stable for at least one year when stored at 2-8 °C and 3 months when stored at 25 °C. (Hazardous Substances Data Bank (HSDB))
Nearby
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